X-Ray Diffraction data from human polymerase eta with incoming dCMPnPP across O4-methyl threofuranosyl thymidine, source of 9CIH structure

Data DOI: 10.15785/SBGRID/1127 | ID: 1127

Publication DOI: 10.1021/acs.biochem.4c00387

9CIH Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Stone Laboratory, Vanderbilt University

Release Date: 13 Sep 2024

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/1127

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/1127 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/1127 .' (Uppsala University, Sweden)

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3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 1127 ; shasum -c files.sha'

Storage requirements: 8.7G

Biological Sample:

human polymerase eta with incoming dCMPnPP across O4-methyl threofuranosyl thymidine

Dataset Type:

X-Ray Diffraction

Subject Composition:

DNA, Protein

Collection Facility:

ESRF ID23-2

Data Creation Date:

2 Dec 2023

Related Datasets:

None


Cite this Dataset

Egli, M; Tomar, R; Stone, MP.. 2024. "X-Ray Diffraction data for: human polymerase eta with incoming dCMPnPP across O4-methyl threofuranosyl thymidine. PDB Code 9CIH", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/1127.

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Dataset Description

Crystal structure of human polymerase eta with incoming dCMPnPP nucleotide opposite O4-methyl threofuranosyl thymidine in DNA template at extension stage

Project Members

Name Additional Roles Affiliation While Working on the Project
Martin EgliDepositorProfessor of Biochemistry (Vanderbilt University)
Michael P. StoneProfessor of Chemistry (Vanderbilt University)
Rachana TomarData CollectorResearch Assistant Professor (Vanderbilt University)
Michael StonePIVanderbilt University

Reprocessing Instructions

Data was indexed, integrated and scaled in HKL2000/ccp4; finally structure was determined in space group P61 at 2.15 Angstrom resolution through molecular replacement.


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.