X-Ray Diffraction data from Mn-bound M230A mutant Nramp/MntH divalent transition metal transporter from Deinococcus radiodurans, source of 8E6I structure

Data DOI: 10.15785/SBGRID/967 | ID: 967

Publication DOI: 10.7554/eLife.84006

8E6I Coordinates: Viewer, PDB (RCSB) (PDBe), MMDB

Gaudet Laboratory, Harvard University

Release Date: 2 May 2023

Data Access Instructions

1. If this dataset is locally available, it should be accessable at /programs/datagrid/967

2. To download this dataset, please run the following command from your Terminal on a Linux or OS X workstation:

'rsync -av rsync://data.sbgrid.org/10.15785/SBGRID/967 .' (Harvard Medical School, USA)

Depending on your location, faster access may be available from a Tier 1 site closer to your location

'rsync -av rsync://sbgrid.icm.uu.se/10.15785/SBGRID/967 .' (Uppsala University, Sweden)

'rsync -av rsync://sbgrid.pasteur.edu.uy/10.15785/SBGRID/967 .' (Institut Pasteur de Montevideo, Uruguay)

'rsync -av rsync://sbgrid.ncpss.org/10.15785/SBGRID/967 .' (Shanghai Institutes for Biological Sciences, China)

3. After the transfer is completed, please issue the following command to verify data integrity:

'cd 967 ; shasum -c files.sha'

Storage requirements: 23G

Biological Sample:

Mn-bound M230A mutant Nramp/MntH divalent transition metal transporter from Deinococcus radiodurans

Dataset Type:

X-Ray Diffraction

Subject Composition:

Ligand, Protein

Collection Facility:

Beamline 23-ID-B with Dectris Eiger-16M pixel detector, NE-CAT, APS, Chicago, IL

Data Creation Date:

20 Sep 2020

Related Datasets:

None


Cite this Dataset

Ray, S; Gaudet, R. 2023. "X-Ray Diffraction data for: Mn-bound M230A mutant Nramp/MntH divalent transition metal transporter from Deinococcus radiodurans. PDB Code 8E6I", SBGrid Data Bank, V1, https://doi.org/10.15785/SBGRID/967.

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Dataset Description

High resolution datasets of Mn-bound M230A mutant collected from 3 separate LCP crystals. Image files that start A131_run2 are from the first crystal, A132_run3 are from the second crystal and A133_run5 are from the third crystal.

Project Members

Name Additional Roles Affiliation While Working on the Project
Shamayeeta RayData Collector, DepositorHarvard University
Rachelle GaudetData Collector, PIHarvard University

Reprocessing Instructions

Data was processed using XDS, indexed using POINTLESS in P22121 space group and scaled using AIMLESS at 2.52A


License and Terms of use

License: CC0

Terms: Our Community Norms as well as good scientific practices expect that proper credit is given via citation. Please use the data citation, as generated by the SBGrid Data Bank.